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Transgenic dog
- 1. Transgenic dog and Transgenic Animals used in vaccine production
- 2. What is transgenic animal? • Animal that carries a foreign gene that has been deliberately inserted into its genome.
- 4. RUPPY –The fluorescent puppy • A team led by Dr.Byeong-chun lee of Seoul National University In south Korea created this fluorescent dog. • Dog genome had helped in making Ruppy. • It is created by cloning fibroplasts cells that expresses a red fluorescent gene produced by sea anemones. • Ruppy is the first transgenic dog.
- 5. Making a glowing dog • Lees team created Ruppy by first infecting dogs fibroplast cells with a virus that inserted a fluorescent gene into its cell nucleus. • Then they transformed the fibroplast nucleus to another dogs egg cell,with its nucleus removed. • After a few hours dividing in a petri dish, researchers implanted cloned embryo into a surrogate mother.
- 6. Growth of embryos 344 embryos implanted into 20 DOGS 7 Pregnancies Five dogs alive healthy and starting to spawn their fluorescent puppies
- 7. Obstacles • Besides the low efficiency of cloning just 1.7% of embryos came to term. • It was difficult to control the nuclear DNA wherein the foreign gene lands. • Lees team used a retrovirus to transfer the fluorescent gene.
- 8. Application of transgenic dogs • Scientists hope to knock out a specific oestrogen receptor in dogs to understand the hormone effects on fertility. • The long life span of dogs and their reproductive cycle could make them more relevant to human fertility than mice.
- 10. Uses of transgenic animals in vaccine production 1)T. Fish -Increased production of growth hormones 2)T. Pig - “Enviro-pigs” better utilisation of phosphorous in feed- solution to animal waste issues. 3)T. Mice - secrets malaria vaccine proteins into their milk. This vaccine protects 80% of monkeys subsequently exposed to malarial parasites.